Abstract
Automated liquid handlers execute digital protocols, but operators must assemble the deck and confirm that the physical setup matches the intended experiment. We present the Labware Setup Checker, a passive vision system that verifies deck preparation on an Opentrons Flex without modifying its hardware or firmware. A consumer webcam mounted outside the robot supplies images to a browser application. The checker segments the deck into slots, corrects slot assignments using the known deck geometry, classifies the labware, and compares the observed setup with a reference protocol library to report missing, misplaced, or unexpected labware. It operates independently of the robot controller and does not interrupt or gate a run. During SwabSeq Respiratory Viral Panel (RVP) pre-PCR deck preparation, the checker tracked a slot from empty to a base and then to the completed plate-and-base assembly, identifying the completed assembly in all 29 frames before robot motion. Across these frames, 95.1% of the 348 labware classifications for slots requiring exact matches agreed with the protocol reference. In 480 frames with partially occluded reservoirs, all 6,720 slot assignments were correct. Across three camera positions and two lighting conditions, slot assignment was 100% correct, and 97.73% of labware classifications matched the protocol reference. Median verification time was 2.10 s on a laptop using only its CPU and 4.24 s from frame capture to receipt of results during AWS deployment in the laboratory. These results show that an external camera and independent software can provide verification of deck setup against protocol requirements within seconds, allowing operators to review the physical setup before starting a run.
Abstract (translated)
URL
https://arxiv.org/abs/2610.02668
